Inactivation of vancomycin-induced unstable L-forms of Staphylococcus aureus by horse serum

نویسندگان

  • Farshad Nojoomi Department of Microbiology, School of Medical Sciences, Military University, Tehran
  • Qorban Behzadian Nejad Department of Microbiology, School of Medical Sciences, Tarbiat Modares University, Tehran
  • Seyed Davar Siadat Department of Microbiology, Pasteur Institute of Iran
چکیده مقاله:

Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance and proliferate in spite of the loss of cell wall is called an L- form. Unstable L- forms  can convert to normal cells but stble L-forms can not.cell wall - deffective state may be induced spontaneously or through the action of an appropriate agent such as antibiotic interfering with the synthesis of cell wall peptidoglycan. Vancomycin is one of these antibiotics which cause L-form formation in some strains of Staphylococcus aureus in presence of osmoprotective compounds. Horse serum and some of organic or mineral materials can be used for better isolation of L- forms in vitro. In this study, standard strain of  Staphylococcus aureus (ATCC 25923) was inoculated  to 2 media: i) L phase medium (LPM) agar containing horse serum & ii) LPM agar without horse serum. Sucrose was used in both above mentioned growth media as osmoprotective and vancomycin was used for inducing L-forms. Microscopic examination revealed presence of small and large colonies on LPM agar lacking horse serum and only large colonies on LPM agar containing horse serum after 3 - 4 days. induced by horse serum were unable to form colony in LPM agar containing horse serum. Results showed that unstable L-forms of Staphylococcus aureus in the presence of horse serum neither can survive nor convert to normal cells.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

inactivation of vancomycin-induced unstable l-forms of staphylococcus aureus by horse serum

substitution at codon ser315 of katg gene, a reliable marker for isoniazid (inh) resistance and proliferate in spite of the loss of cell wall is called an l- form. unstable l- forms can convert to normal cells but stble l-forms can not.cell wall - deffective state may be induced spontaneously or through the action of an appropriate agent such as antibiotic interfering with the synthesis of cell...

متن کامل

Reduced sensitivity of staphylococcus aureus to vancomycin

Introduction: Staphylococcus aureus is one of the most common causes of infections in hospitals and vancomycin is used for the treatment of methicillin resistant S, aureus infections. Therefore, imergence of Staphylococcus aureus with reduced sensitivity to vancomycin is a serious problem. The aim of this study was to investigate the prevalence of Staphylococcus aureus with reduced suscep...

متن کامل

Vancomycin Resistance Pattern of Staphylococcus Aureus among Clinical Samples

Abstract Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus iso...

متن کامل

Prevalence of Vancomycin Resistance in Methicillin-Resistant Staphylococcus Aureus

Background & Aims: Staphylococcus aureus is one of the most common pathogens in nosocomial infections. Vancomycin is the most important therapeutic drug of choice for treatment of infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Therefore, vancomycin-resistant Staphylococcus aureus (VRSA) or vancomycin-intermediate Staphylococcus aureus (VISA) strains are warning...

متن کامل

Prevalence of clindamycin-resistant Staphylococcus aureus induced by macrolide resistance

Introduction: Since the incidence of various infections caused by Staphylococcus aureus increases, the use of effective antibiotics such as clindamycin has been growing to treat the systemic and local infections caused by such organisms. Antibiotic-resistant species are emerging and spreading in today's communities due to misuse of antibiotics and incomplete treatment, turning into a serious ch...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 1  شماره 2

صفحات  77- 81

تاریخ انتشار 2011-12-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023